AZP Dot Assay Kits
anti-Zona Pellucida IgG and IgM Dot Assay kits are intended to be used as a convenient and rapid aid in the analysis of woman infertility.
Atlas Link Technology Co., Ltd is supplying two Dot assay kits for the detection of AZP antibodies, namely AZP IgG test kit and AZP IgM test kit, which are intended to be used to respectively detect antibody isotypes of IgG and IgM specific to the Zona Pellucida, and are intended to be used as a convenient and rapid aid in the analysis of woman infertility.
AZP Testing Principle
anti-Zona Pellucida IgG and IgM Dot Assay kits are rapid test based on the Gold Immuno-filtration Assay (GIFA) principle. In these rapid test kits, purified Zona Pellucida antigen is used as marker, and secondary antibodies, i.e. mouse anti-human IgM, or IgG antibody, conjugated with colloidal gold is used as detector. During the assay test, the IgM or IgG antibody specific to Zona Pellucida, if presents in the serum specimen, antigen+AZP+anti-human-colloidal gold complex will develop in the test spot region, and colored spot will appear, indicating a positive result.
How AZP Causes Infertility
Many immunological reasons have been suggested on how AZP causes infertility. For example, when anti-ZP antibodies present, they can bind with the ZP3, one of the four major zona pellucida glycoproteins which allow species-specific sperm binding, thus will prevent the sperm from binding to the zona pellucida. And when AZP binds with the zona pellucida, the antigen-antibody complex may hinder the sperm from entering the zona pellucida even the sperm binding happen, and block the sperm-ovum interaction and ultimately from fertilizing the ovum.
The zona pellucida is a glycoprotein membrane surrounding the plasma membrane of an oocyte. The zona pellucida is essential for oocytes fertilization. In the process of fertilization, spermatozoa fuse with the zona pellucida which surrounds the mature oocyte; the resulting acrosome reaction breaks down the egg's tough coating so that the sperm can fertilize the ovum. In women body, five days after the fertilization, the blastocyst performs zona hatching; the zona pellucida degenerates and decomposes to be replaced by the underlying layer of Zona Pellucida cells.