ASO IgG Dot Filtration Assay Kit
ASO DOT dot filtration rapid assay kit is a quick test kit for the detection of Anti Streptolysin-O antibody in human serum.
Intended Use
Anti-Streptolysin O (ASO) DOT Assay is used to detect the IgG antibodies specific to anti-Streptolysin O in human serum sample, and is intended to be used as an aid in the diagnosis of current versus past infections with group A streptococci; it also can be used to help to diagnose rheumatic Arthritis.
Testing Principle
In the anti-Streptolysin O (ASO) DOT test cassette, nitrocellulose membrane is pre-coated with Monoclonal Streptolysin O antigen. During the assay, the anti-Streptolysin O IgG antibody, if present in the serum specimen, will be captured by the pre-coated ASO antigen. In the subsequent procedure, the reagent of anti-human monoclonal IgG antibody (anti-human) conjugated with colloidal gold is added. This secondary antibody will be captured by the ASO IgG antibody bound to the membrane in previous step, and a colored dot will develop, indicating a positive result.
ASO Antibody and Rheumatoid Arthritis
When Lancefield group A streptococci infect human body, they produce many enzymes, including oxygen labile streptolysin-O and oxygen stable streptolysin-S. Both the enzymes are involved in incurring of hemolysis, especially in beta-hemolysis. When human body is infected with streptococci, it produces antibodies against these two antigens. Anti-streptolysin O (ASO) is the antibody produced against streptolysin O antigen. An elevated or rising level indicate a past or present infection of streptococci. Until now, ASO is one of the first bacterial markers used for diagnosis and follow up of rheumatism or scarlet fever.
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