Fentanyl Urine Test Kits

Fentanyl Rapid Test kit (Urine) is a rapid visual immunoassay for the qualitative, presumptive detection of Fentanyl in human urine specimens at the cut-off concentration of 200 ng/mL.

Fentanyl Urine Test strip and Cassette

Principle of FYL Urine Test

FYL Rapid Test Strip (Urine) detects Fentanyl through visual interpretation of color development on the test strip. Fentanyl drug conjugates are immobilized on the test region of the membrane. During FYL testing, the specimen reacts with antibodies conjugated to colored particles and precoated on the sample pad. The mixture then migrates through the membrane under capillary action, and interacts with reagents on the membrane.

If there are insufficient fentanyl molecules in the specimen, the antibody-colored particle conjugate will bind to the drug conjugates on the testing region on the FYL test strip, forming a colored band at the test region of the membrane. Therefore, the development of a colored band in the test region indicates a negative fentanyl testing result.

If fentanyl molecules are present in the urine with a higher concentration than the cut-off of this FYL urine screening kit, they compete with the drug conjugate on the test region for limited antibody binding sites. This will prevent attachment of the antibody-colored particle conjugate to the test region. Therefore, the absence of a colored band at the test region indicates a positive fentanyl testing result.

The appearance of a colored band at the control region serves as a procedural control, indicating that the proper volume of specimen has been added and membrane wicking effect has occurred.

Reagents Used in FYL Test Strip

FYL Urine Test kit comes in both strip style and cassette style. Each fentanyl test device consists of a reagent strip. The amount of each antibody to fentanyl coated on the strip is less than 0.001 mg for antigen conjugates and goat anti-rabbit IgG antibodies, and less than 0.0015 mg for antibody components.

Fentanyl in Recreation Use

Fentanyl is a synthetic opioid related to the phenyl piperidines. Fentanyl is approximately 100 times more potent than morphine. This agent is highly lipid soluble, and can penetrate rapidly cross the blood-brain barrier. This is reflected in the half-life for equilibration between the plasma and cerebrospinal fluid of approximately 5 minutes for fentanyl. The levels in plasma and cerebrospinal fluid decline rapidly owing to redistribution of fentanyl from highly perfused tissue groups to other tissues, such as muscle and fat. As saturation of less well-perfused tissue occurs, the duration of effect of fentanyl and sufentanil approaches the length of their elimination half-lives of between 3 and 4 hours. Fentanyl undergoes hepatic metabolism and renal excretion. Therefore, with the use of higher doses or prolonged infusions, fentanyl becomes longer acting.

Cut-off of Drug Test Kits

No. Abbr. Full Name Cut-off (ng/ml)
1 AMP Amphetamine 1000
2 BAR SecoBarbital 300
3 BUP Buprenorphine 10
4 BZO Oxazepam Benzodiazepines 300
5 COC Cocaine 300
6 COT Cotinine 200
7 EDDP ethylenediamine-dimethyl phosphinic acid 100
8 FYL Fentanyl 200
9 K2 Synthetic Cannabis 50
10 KET Ketamine 1000
11 LSD Lysergic acid diethylamide 50
12 MDMA 3,4-methylenedioxy methamphetamine 500
13 MET Methamphetamine 1000
14 MOR Morphine 300
15 MQL Methaqualone 300
16 MTD Methadone 300
17 OPI Opiates Morphine 2000
18 OXY Oxycodone 100
19 PCP Phencyclidine 25
20 PPX Propoxyphene 300
21 TCA Tricyclic Antidepressants 1000
22 THC Marijuana 50
23 TML Tramadol 100

Add EDDP Test Kits to Inquiry Cart

How to Use
Cat. No. Description (& Details) Specimen PCS/Box Quan. per CTN Quantity (Tests)
C8111 Methadone Metabolite (EDDP) Urine Strip Urine100 Bulk: 6000 PCS
Boxed: 48 Boxes
C8112 Methadone Metabolite (EDDP) Urine Cassette Urine25 Bulk: 1600 PCS
Boxed: 40 Boxes