anti-TP ELISA Kit
Anti-TP ELISA kit is an enzyme-linked immunosorbent assay, intended for screening of blood donors, and as an aid for the diagnosis and management of clinical conditions known as syphilis.
Anti-TP ELISA Kit
This anti-TP ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for qualitative determination of the antibodies to Treponema pallidum (TP) in human serum or plasma. It is intended for screening of blood donors and as an aid for the diagnosis and management of clinical conditions known as syphilis.
Assay Principle of Anti-TP ELISA
With this Syphilis ELISA kit, the detection of anti-TP antibodies is achieved by antigen ”sandwich” enzyme-linked method, where polystyrene micro-well strips are pre-coated with recombinant Treponema pallidum antigens expressed in E. coli. The sample is incubated in the micro-wells together with recombinant TP antigens conjugated to horseradish peroxidase (HRP-Conjugate). The pre-coated antigens express the same epitopes as the HRP-Conjugate antigens, but are expressed in different hosts. In case of presence of anti-TP in the sample, during incubation the pre-coated and conjugated antigens will be bound to the two variable domains of the antibody and the specific antigens-antibody immunocomplex is captured on the solid phase.
After washing to remove sample and unbound conjugates, Chromogens solutions containing tetramethylbenzidine (TMB) and urea peroxide are added into the wells. In presence of the antigen-antibody-antigen “sandwich” complex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue-colored product. The blue color turns yellow after stopping the reaction with sulfuric acid.
The amount of color can be measured and is proportional to the amount of antibody in the sample. Wells containing samples negative for anti-TP remain colorless.
Syphilis and TP Virus
Syphilis is a disease caused by Spirochete bacterium called Treponema pallidum (TP). If untreated, the organisms move throughout the body and can cause damage to many organs, making syphilis a life-threatening disease if not treated early enough.
The first detectable response to infection is the production of specific anti-treponemal IgM, which can be detected within 4 to 7 days after the chancre appears and until the end of the second week of infection; anti-treponemal IgG appears at about four weeks later. By the time that symptoms develop, most patients have detectable IgG and IgM.