Streptolysin-O IgG Dot Assay
Streptolysin-O IgG Dot Assay kit (链球菌(ASO)IgG抗体渗滤法检测试剂) is a two-step qualitative dot filtration rapid diagnostic test kit, based on the Colloidal Gold Immuno-filtration indirect principle, which is used to detect Streptolysin-O IgG Antibody in human Serum specimen.
Detailed information for Streptolysin-O IgG Dot Assay is provided below, including:
- General and Technical information of this ASO test kit;
- Packing specification of this ASO DOT Card style test kit (gross weight in full kit package and in bulk packing);
- Specimen used to carry out the assay, and the requirements on specimen collection, storage and processing; and,
- Specific immuno assay principle used in this Anti-Streptolysin-O Test Kit.
Main technical information of this ASO IgG test kit is listed in the following table:
|Catalog No.:||ASO R222|
|Description:||Streptolysin-O IgG Dot Assay|
|No. of Step:||Two Step|
|Reading Time:||3 Minutes|
|Quan. or Qual.:||Qualitative|
|Assay Principle:||Colloidal Gold Immuno-filtration indirect|
The standard kit size of Anti-Streptolysin-O DOT Card test kit is 25 tests per box; 36 boxes per carton.
To save freight cost, sometimes bulk packing is used, meaning test strip, cassette or multi-test panel is packed in single aluminum pouch; 50 or 100 pouches in one plastic bag; and then plastic bags are packed in cartons along with the unfolded boxes for the test kits. In this way, the customers can easily pack the tests into test kits after receiving the bulk packed goods.
|Tests per Kit:||25 Tests|
|Dimension of Kit Box:||21.0 * 12.5 * 6.5 CM|
|Boxes per Carton:||36 Kits|
|Tests per Carton (BULK):||1200 Tests|
|G.W. per Carton (Boxed):||15.0 KG|
|G.W. per Carton (Bulk):||15.0 KG|
|Carton Dimension:||53 * 45 * 36 CM|
|Carton Volume:||0.085 CBM|
- Filtration assay device;
- Washing buffer Gold colloidal Conjugate solution;
- Disposable dropper for sample transfer;
- Instruction For Use (IFU).
To carry out the assay with a Streptolysin-O IgG Dot Assay, Serum should be collected according to the instructions given in the Instruction For Use included in the test kit. After collecting, it is preferable to perform the testing as early as possible; if not viable, the specimen should be stored properly. A general introduction about the collection, and storage of the specimens is given below.
Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.
Allow the blood to clot, or separate the serum by centrifugation.
Carefully withdraw the serum into a new pre-labeled tube.
Serum Storage Condition
Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.
Colloidal Gold Immuno-filtration indirect method is used in this Streptolysin-O IgG Dot Assay to detect the Streptolysin-O IgG Antibody in human Serum specifically. The introduction of this Colloidal Gold Immuno-filtration indirect testing principle is given blow.
With indirect Dot filtration test kit, the millipore filtering membrane is pre-coated with purified or recombinant viral antigens. The detecting conjugate reagent is stored in a vial, whose main component is rabbit anti human IgG (anti-IgG) antibodies, which is conjugated with colloidal gold particles.
During the first step, sample is added to the sample well in the test cassette. The target IgG antibodies, if present, will be captured by the pre-coated antigens.
The wells are washed to remove unbound serum proteins. Then, the colloidal gold conjugate is added. If the target IgG antibodies have been captured in the first step, the IgG antibody will react with the added secondary antibody, anti-IgG conjugate, and red color will develop, indicating a positive result.