Anti-Zona Pellucida Dot Assay Kits & ELISA Kits

Anti-Zona Pellucida IgG DOT Assay Kit

Atlas Link Technology Co., Ltd is supplying two Dot assay kits for the detection of AZP antibodies, namely anti-Zona Pellucida IgG test kit and anti-Zona Pellucida IgM test kit, which are intended to be used to detect the Zona Pellucida specific antibody isotypes of IgM and IgG respectively, as are intended to be used as a convenient and rapid aid in the analysis of woman infertility.

The AZP DOT assay reagent kits are rapid test based on the Gold Immuno-filtration Assay (GIFA) principle. In this kit, purified Zona Pellucida antigen is used as marker, and secondary antibodies, i.e. mouse anti-human IgM, or IgG antibody, conjugated with colloidal gold is used as detector. During the assay test, the IgM or IgG antibody specific to Zona Pellucida, if presents in the serum specimen, antigen+AZP+anti-human-colloidal gold complex will develop in the test spot region, and colored spot will appear, indicating a positive result. If no AZP presents in the specimen, the colloidal gold particle will not be bound on the test spot region, and no color develops there, indicating a negative result.

Anti-Zona Pellucida (AZP) and Infertility

The zona pellucida is a glycoprotein membrane surrounding the plasma membrane of an oocyte. The zona pellucida is essential for oocytes fertilization. In the process of fertilization, spermatozoa fuse with the zona pellucida which surrounds the mature oocyte; the resulting acrosome reaction breaks down the egg's tough coating so that the sperm can fertilize the ovum.

In humans, five days after the fertilization, the blastocyst performs zona hatching; the zona pellucida degenerates and decomposes to be replaced by the underlying layer of Zona Pellucida cells. Many immunological reasons have been suggested on how AZP causes infertility. For example, when anti-ZP antibodies present, they can bind with the ZP3, one of the four major zona pellucida glycoproteins which allow species-specific sperm binding, thus will prevent the sperm from binding to the zona pellucida. And when AZP binds with the ZP, the antigen-antibody complex may hinder the sperm from entering the zona pellucida even the sperm binding happen, and block the sperm-ovum interaction and ultimately from fertilizing the ovum.

Anti-Zona Pellucida Immunoassay Kits

No.Cat. No.Description
(Click For Details)
SpecimenPCS/BoxQuan. per CTN Quantity Interested* Add to
1 AZP I712 Anti-Zona Pellucida IgM Dot Assay serum 25 Bulk: 1200 PCS
Boxed: 36 Boxes
2 AZP I722 Anti-Zona Pellucida IgG Dot Assay serum 25 Bulk: 1200 PCS
Boxed: 36 Boxes
3 AZP I714 Anti-Zona Pellucida IgM ELISA Kit serum 48/96T Boxed: 40 Kits boxes
4 AZP I724 Anti-Zona Pellucida IgG ELISA Kit serum 48/96T Boxed: 40 Kits boxes
5 AZP I734 Anti-Zona Pellucida IgA ELISA Kit serum 48/96T Boxed: 40 Kits boxes
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