Mycoplasma Pneumoniae IgG DOT-ELISA

Mycoplasma Pneumoniae IgG DOT-ELISA (肺炎支原体(MP)IgG抗体检测试剂盒) is a two-step qualitative rapid diagnostic test kit, based on the Indirect Dot ELISA principle, which is used to detect Mycoplasma Pneumoniae IgG Antibody in human Serum specimen.

Detailed information for Mycoplasma Pneumoniae IgG DOT-ELISA is provided below, including:

  1. General and Technical information of this MP test kit;
  2. Packing specification of this MP DOT ELISA style test kit (gross weight in full kit package and in bulk packing);
  3. Specimen used to carry out the assay, and the requirements on specimen collection, storage and processing; and,
  4. Specific immuno assay principle used in this Mycoplasma Pneumonia Test Kit.

Main technical information of this MP IgG test kit is listed in the following table:

Catalog No.:MP 8423
Description:Mycoplasma Pneumoniae IgG DOT-ELISA
Category:Respiratory Tract Disease
No. of Step:Two Step
Reading Time:10-2-2-3 Minutes
Cut Off:
Quan. or Qual.:Qualitative
Assay Principle:Indirect Dot ELISA

The standard kit size of this Mycoplasma Pneumonia DOT ELISA test kit is 48 tests per box; 42 boxes per carton.

To save freight cost, sometimes bulk packing is used, meaning test strip, cassette or multi-test panel is packed in single aluminum pouch; 50 or 100 pouches in one plastic bag; and then plastic bags are packed in cartons along with the unfolded boxes for the test kits. In this way, the customers can easily pack the tests into test kits after receiving the bulk packed goods.

Tests per Kit:48 Tests
Dimension of Kit Box:13.5 * 9.0 * 8.0 CM
Boxes per Carton:42 Kits
Tests per Carton (BULK):42 Tests
G.W. per Carton (Boxed):15.0 KG
G.W. per Carton (Bulk):15.0 KG
Carton Dimension:55 * 44 * 35 CM
Carton Volume:0.084 CBM

Materials Provided

  • Immuno-filtration device in pouch with desiccant, with an integrated sample flow controller;
  • HRP-conjugate: 1 dropper bottle of 2ml;
  • Sample preservation buffer: 6ml;
  • Wash Buffer: 1 dropper bottle of 6ml;
  • Chromogen Solution: 1 bottle of 1ml;
  • Stop Solution: 1 dropper bottle of 1ml;
  • Sample collection tube: 6 tubes/kit;
  • Sample Lysis Bottle: 6 pieces/kit;
  • Measuring Pipette: 6 pieces/kit;
  • Plastic sealable bag: 1pcs (store the cards not in use);
  • Instruction For Use (IFU): 1 copy.

To carry out the assay with a Mycoplasma Pneumoniae IgG DOT-ELISA, Serum should be collected according to the instructions given in the Instruction For Use included in the test kit. After collecting, it is preferable to perform the testing as early as possible; if not viable, the specimen should be stored properly. A general introduction about the collection, and storage of the specimens is given below.

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

Serum Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 5 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Indirect Dot ELISA method is used in this Mycoplasma Pneumoniae IgG DOT-ELISA to detect the Mycoplasma Pneumoniae IgG Antibody in human Serum specifically. The introduction of this Indirect Dot ELISA testing principle is given blow.

Dot ELISA Indirect Method Illustration

IgG-class antibodies usually are connected with secondary infections, and will usually remain in the human body fluid for a long time. Usually, indirect method is used to test this class of antibodies.

In indirect dot ELISA kit, millipore filtering membrane (usually nitrocellulose,NC) is pre-coated with recombinant or purified antigen, which acts as capturer. And a secondary antibody, such as goat anti-mouse IgG antibody (anti-IgG), is made into conjugate reagent by conjugate this second antibody with horseradish peroxidase (HRP). This conjugate acts as indicator.

When the patient’s fluid sample is added to the membrane, it will filtrate through the latter. During the minutes of filtration process, the IgG and other isotypes of antibodies, if present, will be captured by the immobilized antigen. The unbound specimen is washed away completely after wash buffer is added.

Then, anti-IgG conjugate reagent is added, which will specifically interact with the captured IgG antibodies.

And Ag-ab-anti-IgG complex will develop, at which end is the HRP.

After washing to remove unbound conjugates, Chromogen solutions are added to the membrane, then stop solution is added. When HRP presents, the colorless chromogen is hydrolyzed to a blue color product at the testing dot area, indicating the specimen is IgG positive.