Influenza-A IGM Antibody Dot Assay
Influenza-A IGM Antibody Dot Assay kit (A型流感IGM抗体渗滤法快速诊断试剂盒) is a two-step qualitative dot filtration rapid diagnostic test kit, based on the Colloidal Gold Immuno-filtration Double Antibody Sandwich principle, which is used to detect IgM antibody to Influenza A virus in human Serum or Plasma specimen.
Detailed information for Influenza-A IGM Antibody Dot Assay is provided below, including:
- General and Technical information of this FLU A test kit;
- Packing specification of this FLU A DOT Card style test kit (gross weight in full kit package and in bulk packing);
- Specimen used to carry out the assay, and the requirements on specimen collection, storage and processing; and,
- Specific immuno assay principle used in this Influenza A & B Test Kit.
Main technical information of this Influenza A IGM test kit is listed in the following table:
|Catalog No.:||FLU 8312|
|Description:||Influenza-A IGM Antibody Dot Assay|
|Category:||Respiratory Tract Disease|
|No. of Step:||Two Step|
|Reading Time:||3 Minutes|
|Quan. or Qual.:||Qualitative|
|Assay Principle:||Colloidal Gold Immuno-filtration Double Antibody Sandwich|
The standard kit size of this Influenza A & B DOT Card test kit is 25 tests per box; 36 boxes per carton.
To save freight cost, sometimes bulk packing is used, meaning test strip, cassette or multi-test panel is packed in single aluminum pouch; 50 or 100 pouches in one plastic bag; and then plastic bags are packed in cartons along with the unfolded boxes for the test kits. In this way, the customers can easily pack the tests into test kits after receiving the bulk packed goods.
|Tests per Kit:||25 Tests|
|Dimension of Kit Box:||21.0 * 12.5 * 6.5 CM|
|Boxes per Carton:||36 Kits|
|Tests per Carton (BULK):||1200 Tests|
|G.W. per Carton (Boxed):||15.0 KG|
|G.W. per Carton (Bulk):||15.0 KG|
|Carton Dimension:||53 * 45 * 36 CM|
|Carton Volume:||0.085 CBM|
- Filtration assay device;
- Washing buffer Gold colloidal Conjugate solution;
- Disposable dropper for sample transfer;
- Instruction For Use (IFU).
To carry out the assay with a Influenza-A IGM Antibody Dot Assay, Serum or Plasma should be collected according to the instructions given in the Instruction For Use included in the test kit. After collecting, it is preferable to perform the testing as early as possible; if not viable, the specimen should be stored properly. A general introduction about the collection, and storage of the specimens is given below.
Collect blood specimen into a lavender, blue or green top blood collection tube, containing EDTA, citrate or heparin, respectively, by vein puncture.
Separate the plasma by centrifugation.
Carefully withdraw the plasma into a new pre-labeled tube.
Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.
Allow the blood to clot, or separate the serum by centrifugation.
Carefully withdraw the serum into a new pre-labeled tube.
Serum, Plasma Storage Condition
Test specimens as soon as possible after collecting. The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.
Colloidal Gold Immuno-filtration Double Antibody Sandwich method is used in this Influenza-A IGM Antibody Dot Assay to detect the IgM antibody to Influenza A virus in human Serum or Plasma specifically. The introduction of this Colloidal Gold Immuno-filtration Double Antibody Sandwich testing principle is given blow.
With this double antibody test, the detection target usually is hormone protein or viral antigen.
In this kind of dot filtration immunoassay test kit, monoclonal or polyclonal antibodies specific to the target protein or antigen are used as capturer, which are pre-coated in a dot area in the millipore filtering membrane, which called test region. And another antigen or secondary antibody, such as mouse anti-goat IgG antibodies, is coated in another dot area, which is supposed to develop visual red dot regardless of the testing results. This is the mechanism to insure that the procedure is correctly followed, and the reagent is not deteriorated.
When the sample is added, the antigen, if present, will react with the pre-coated antibody, and bounded there.
After washing the membrane with diluents, the antibody colloidal gold conjugate is added. The antibody will bind to another site on the antigen, which is captured in the previous step. When the antibody-antigen-antibody complex develops, the conjugated colloidal gold particles will congregate and develop red or pink color, indicating a positive result.
If there is no target antigen or viral protein in the sample, the complex will not develop, the colloidal gold particles will not gather, and no color will develop, indicating a negative result.