Vibrio Cholera O1 Rapid Test

Vibrio Cholera O1 Rapid Test (霍乱O1病原测试卡) is a one-step qualitative lateral flow rapid diagnostic test kit, based on the Colloidal Gold Chromatographic Immuno Double Antibody Sandwich principle, which is used to detect Vibrio Cholera O1 Antigens in human Feces or Environment Water specimen.

Detailed information of Vibrio Cholera O1 Rapid Test

  1. General and Technical information of this VCO1 test kit;
  2. Packing specification of this VCO1 Card style test kit (gross weight in full kit package and in bulk packing);
  3. Specimen used to carry out the assay, and the requirements on specimen collection, storage and processing; and,
  4. Specific immuno assay principle used in this Salmonella Typhi Test Kit.

Main technical information of this Vibrio Cholera test kit is listed in the following table:

Catalog No.:VCO1 951
Description:Vibrio Cholera O1 Rapid Test
Category:Digestive Track Disease
Style:Card
Unit:PCS
Specimen:Feces, Environment Water
No. of Step:One Step
Reading Time:15 Minutes
Cut Off:10^4 CFU/ml
Specification:4.0 mm
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Assay Principle:Colloidal Gold Chromatographic Immuno Double Antibody Sandwich

The standard kit size of this Salmonella Typhi Card test kit is 25 tests per box; 40 boxes per carton.

To save freight cost, sometimes bulk packing is used, meaning test strip, cassette or multi-test panel is packed in single aluminum pouch; 50 or 100 pouches in one plastic bag; and then plastic bags are packed in cartons along with the unfolded boxes for the test kits. In this way, the customers can easily pack the tests into test kits after receiving the bulk packed goods.


Tests per Kit:25 Tests
Dimension of Kit Box:21.0 * 12.5 * 6.5 CM
Boxes per Carton:40 Kits
Tests per Carton (BULK):1600 Tests
G.W. per Carton (Boxed):14.0 KG
G.W. per Carton (Bulk):16.5 KG
Carton Dimension:53 * 45 * 36 CM
Carton Volume:0.085 CBM

Materials Provided

  • Test cassette in single foil pouch with desiccant;
  • Disposable sample dropper;
  • Instruction leaflet.

To carry out the assay with a Vibrio Cholera O1 Rapid Test, Feces or Environment Water should be collected according to the instructions given in the Instruction For Use included in the test kit. After collecting, it is preferable to perform the testing as early as possible; if not viable, the specimen should be stored properly. A general introduction about the collection, and storage of the specimens is given below.

Feces Collection

Collect a random feces sample in a clean, dry container.

Insert the stick or dropper into the feces to get about 0.5g or 0.3ml feces sample.

Add feces sample to the vial of Sample Buffer A, and mix the feces sample with Sample Buffer A thoroughly.

The supernatant will be used for the test.

Environment Water Collection

Collect environment water sample with a clean and dry container, which is ready for the assay immediately.

Feces, Environment Water Storage Condition

Test specimens as soon as possible after collecting.

The specimens can be stored up to 3 days at 2-8°C. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times.

Prior to testing, bring frozen specimens to room temperature slowly.

Colloidal Gold Chromatographic Immuno Double Antibody Sandwich method is used in this Vibrio Cholera O1 Rapid Test to detect the Vibrio Cholera O1 Antigens in human Feces or Environment Water specifically. The introduction of this Colloidal Gold Chromatographic Immuno Double Antibody Sandwich testing principle is given blow.

GICA Double Antibody Sandwich Method Illustration

In the double antibody sandwich lateral flow chromatographic rapid test, two antibodies are used to detect viral protein or antigen.

In the test band region, antibody specific to the target antigen or protein is immobilized. In the end of the membrane strip where the samples are added, another antibody conjugated with colloidal gold particles is embedded.

During the testing process, specimen from the patient is added to the end of the membrane strip. The antibody conjugate particles will resolve into the specimen, where the antibody conjugate will react and bound to the target protein or viral antigen, if present.

This antigen-antibody conjugate complex then moves along the strip to the testing band region, under capillary effect. The immobilized antibody in the test line region then bound to another binding site on the antigen complex, and the antibody-antigen-antibody complex is fixed on test band region.

When the complex congregates, the highly concentrated colloidal gold particles will develop some red or pink color, indicate a positive result.

If there is no target protein or antigen in the specimen, the colloidal gold particles will not congregate and no color will develop, indicating a negative result.