HIV 1+2 Ag/Ab ELISA Kit
HIV 1+2 Ag/Ab ELISA Kit (艾滋1+2型抗原抗体诊断试剂盒) is a two-step, qualitative , enzyme-linked immunosorbent assay (ELISA) test kit, based on the Double antigen and Double antibody sandwich ELISA principle, which is used to detect HIV1 and HIV2 Antigens Antibodies in human Serum or Plasma specimen.
Please refer to the Instruction for Use (IFU) of the HIV 1+2 Ag/Ab ELISA Kit.
(IFUs are subject to changes without prenotice; reading the package insert before carrying out any assay, it is always a good protocol for laboratory activity.)
Detailed information of HIV 1+2 Ag/Ab ELISA Kit
- General and Technical information of this HIV test kit;
- Packing specification of this Microwell style HIV test kit (gross weight and carton dimension etc.);
- Specimen used to carry out the assay, and the requirements on specimen collection, storage and processing; and,
- Specific immuno assay principle used in this HIV 1/2 Test Kit.
Main technical information of this HIV Ab+Ag Microwell test kit is listed in the following table:
|Catalog No.:||HIV 3124|
|Description:||HIV 1+2 Ag/Ab ELISA Kit|
|Category:||Sexually Transmitted Disease|
|No. of Step:||Two Step|
|Reading Time:||60-30-15 Minutes|
|Quan. or Qual.:||Qualitative|
|Assay Principle:||Double antigen and Double antibody sandwich ELISA|
The standard kit size of HIV 1/2 Microwell Test Kit is 96 Tests per box; 40 boxes per carton; 48T and 480T packing specifications are available for choice.
|Tests per Kit:||96 Tests|
|Dimension of Kit Box:||13.5 * 9.0 * 8.0 CM|
|Boxes per Carton:||40 Kits|
|G.W. per Carton:||15.0 KG|
|Carton Dimension:||59 * 45 * 38 CM|
|Carton Volume:||0.100 CBM|
- MICROWELL PLATE: 1 plate;
- NEGATIVE CONTROL: 1 vial;
- POSITIVE CONTROL SERUM: 1 vial;
- SPECIMEN DILUENT: 1 vial;
- HRP-CONJUGATE REAGENT: 1 vial;
- WASH BUFFER: 1 bottle;
- CHROMOGEN SOLUTION A: 1 vial;
- CHROMOGEN SOLUTION B: 1 vial;
- STOP SOLUTION: 1 vial;
- PLASTIC SEALABLE BAG: 1 piece;
- CARDBOARD PLATE COVER;
- PACKAGE INSERT: 1 copy.
To carry out the assay with a HIV 1+2 Ag/Ab ELISA Kit, Serum or Plasma should be collected according to the instructions given in the Instruction For Use included in the test kit. After collecting, it is preferable to perform the testing as early as possible; if not viable, the specimen should be stored properly. A general introduction about the collection, and storage of the specimens is given below.
Collect blood specimen into a lavender, blue or green top blood collection tube, containing EDTA, citrate or heparin, respectively, by vein puncture.
Separate the plasma by centrifugation.
Carefully withdraw the plasma into a new pre-labeled tube.
Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.
Allow the blood to clot, or separate the serum by centrifugation.
Carefully withdraw the serum into a new pre-labeled tube.
Serum, Plasma Storage Condition
Test specimens as soon as possible after collecting. The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.
Double antigen and Double antibody sandwich ELISA method is used in this HIV 1+2 Ag/Ab ELISA Kit to detect the HIV1 and HIV2 Antigens Antibodies in human Serum or Plasma specifically. The introduction of this Double antigen and Double antibody sandwich ELISA testing principle is given blow.
A simple combination of double antigen sandwich and double antibody sandwich in a single test kit.