Anti-HCV ELISA Kit

Anti-HCV ELISA Kit (丙型肝炎抗体诊断试剂盒) is a two-step, qualitative , enzyme-linked immunosorbent assay (ELISA) test kit, based on the Indirect enzyme-linked immunosorbent assay (ELISA) principle, which is used to detect Antibodies to hepatitis C in human Serum or Plasma specimen.

Please refer to the Instruction for Use (IFU) of the Anti-HCV ELISA Kit.
(IFUs are subject to changes without prenotice; reading the package insert before carrying out any assay, it is always a good protocol for laboratory activity.)

Detailed information of Anti-HCV ELISA Kit

  1. General and Technical information of this HCV test kit;
  2. Packing specification of this Microwell style HCV test kit (gross weight and carton dimension etc.);
  3. Specimen used to carry out the assay, and the requirements on specimen collection, storage and processing; and,
  4. Specific immuno assay principle used in this Hepatitis C Test Kit.

Main technical information of this Anti-HCV Microwell test kit is listed in the following table:

Catalog No.:HCV 2714
Description:Anti-HCV ELISA Kit
Category:Hepatitis Disease
Style:Microwell
Unit:Box
Specimen:Serum, Plasma
No. of Step:Two Step
Reading Time:30-30-15 Minutes
Cut Off:
Specification:48T/96T/480T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Assay Principle:Indirect enzyme-linked immunosorbent assay (ELISA)

The standard kit size of Hepatitis C Microwell Test Kit is 96 Tests per box; 40 boxes per carton; 48T and 480T packing specifications are available for choice.


Tests per Kit:96 Tests
Dimension of Kit Box:13.5 * 9.0 * 8.0 CM
Boxes per Carton:40 Kits
G.W. per Carton:15.0 KG
Carton Dimension:59 * 45 * 38 CM
Carton Volume:0.100 CBM

Materials Provided

  • MICROWELL PLATE: 1 plate;
  • NEGATIVE CONTROL: 1 vial;
  • POSITIVE CONTROL SERUM: 1 vial;
  • SPECIMEN DILUENT: 1 vial;
  • HRP-CONJUGATE REAGENT: 1 vial;
  • WASH BUFFER: 1 bottle;
  • CHROMOGEN SOLUTION A: 1 vial;
  • CHROMOGEN SOLUTION B: 1 vial;
  • STOP SOLUTION: 1 vial;
  • PLASTIC SEALABLE BAG: 1 piece;
  • CARDBOARD PLATE COVER;
  • PACKAGE INSERT: 1 copy.

To carry out the assay with a Anti-HCV ELISA Kit, Serum or Plasma should be collected according to the instructions given in the Instruction For Use included in the test kit. After collecting, it is preferable to perform the testing as early as possible; if not viable, the specimen should be stored properly. A general introduction about the collection, and storage of the specimens is given below.

Plasma Collection

Collect blood specimen into a lavender, blue or green top blood collection tube, containing EDTA, citrate or heparin, respectively, by vein puncture.

Separate the plasma by centrifugation.

Carefully withdraw the plasma into a new pre-labeled tube.

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

Serum, Plasma Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Indirect enzyme-linked immunosorbent assay (ELISA) method is used in this Anti-HCV ELISA Kit to detect the Antibodies to hepatitis C in human Serum or Plasma specifically. The introduction of this Indirect enzyme-linked immunosorbent assay (ELISA) testing principle is given blow.

ELISA Indirect Method Illustration

With indirect ELISA kit, polystyrene microwell strips are pre-coated with purified or recombinant viral antigens.

The antibody detection is achieved in two steps. During the first incubation step, the target antibodies, if present, will be bound to the solid phase pre-coated antigens.

The wells are washed to remove unbound serum proteins, and rabbit anti-human IgG antibodies (anti-IgG) conjugated to horseradish peroxidase (HRP) are added. During the second incubation step, these HRP-conjugated antibodies will be bound to any antigen-antibody complexes previously formed.

Then the unbound HRP-conjugate is then removed by washing. Chromogen solutions containing Tetramethyl benzidine (TMB) and urea peroxide are added to the wells. In presence of the antigen-antibody-anti-IgG (HRP) immuno complex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue colored product which turns blue color turns yellow after stopping the reaction with sulfuric acid, indicating a positive result.

Wells containing samples negative for the target antibodies remain colorless.