HBcAb ELISA Kit
HBcAb ELISA Kit (乙肝C抗体HBcAb检测试剂盒) is a one-step, qualitative , enzyme-linked immunosorbent assay (ELISA) test kit, based on the Capture enzyme-linked immunosorbent assay (ELISA) principle, which is used to detect Anti Hepatitis-B Core antigen in human Serum or Plasma specimen.
Please refer to the Instruction for Use (IFU) of the HBcAb ELISA Kit.
(IFUs are subject to changes without prenotice; reading the package insert before carrying out any assay, it is always a good protocol for laboratory activity.)
Detailed information of HBcAb ELISA Kit
- General and Technical information of this HBc test kit;
- Packing specification of this Microwell style HBc test kit (gross weight and carton dimension etc.);
- Specimen used to carry out the assay, and the requirements on specimen collection, storage and processing; and,
- Specific immuno assay principle used in this Hepatitis B Test Kit.
Main technical information of this HBcAb Microwell test kit is listed in the following table:
|Catalog No.:||HBV 2514|
|Description:||HBcAb ELISA Kit|
|No. of Step:||One Step|
|Reading Time:||60-15 Minutes|
|Quan. or Qual.:||Qualitative|
|Assay Principle:||Capture enzyme-linked immunosorbent assay (ELISA)|
The standard kit size of Hepatitis B Microwell Test Kit is 96 Tests per box; 40 boxes per carton; 48T and 480T packing specifications are available for choice.
|Tests per Kit:||96 Tests|
|Dimension of Kit Box:||13.5 * 9.0 * 8.0 CM|
|Boxes per Carton:||40 Kits|
|G.W. per Carton:||15.0 KG|
|Carton Dimension:||59 * 45 * 38 CM|
|Carton Volume:||0.100 CBM|
- MICROWELL PLATE: 1 plate;
- NEGATIVE CONTROL: 1 vial;
- POSITIVE CONTROL SERUM: 1 vial;
- SPECIMEN DILUENT: 1 vial;
- HRP-CONJUGATE REAGENT: 1 vial;
- WASH BUFFER: 1 bottle;
- CHROMOGEN SOLUTION A: 1 vial;
- CHROMOGEN SOLUTION B: 1 vial;
- STOP SOLUTION: 1 vial;
- PLASTIC SEALABLE BAG: 1 piece;
- CARDBOARD PLATE COVER;
- PACKAGE INSERT: 1 copy.
To carry out the assay with a HBcAb ELISA Kit, Serum or Plasma should be collected according to the instructions given in the Instruction For Use included in the test kit. After collecting, it is preferable to perform the testing as early as possible; if not viable, the specimen should be stored properly. A general introduction about the collection, and storage of the specimens is given below.
Collect blood specimen into a lavender, blue or green top blood collection tube, containing EDTA, citrate or heparin, respectively, by vein puncture.
Separate the plasma by centrifugation.
Carefully withdraw the plasma into a new pre-labeled tube.
Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.
Allow the blood to clot, or separate the serum by centrifugation.
Carefully withdraw the serum into a new pre-labeled tube.
Serum, Plasma Storage Condition
Test specimens as soon as possible after collecting. The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.
Capture enzyme-linked immunosorbent assay (ELISA) method is used in this HBcAb ELISA Kit to detect the Anti Hepatitis-B Core antigen in human Serum or Plasma specifically. The introduction of this Capture enzyme-linked immunosorbent assay (ELISA) testing principle is given blow.
For the detection of IgM-class antibodies, in order to avoid the interference from the IgG class, a solid phase, two-step incubation, antibody capture assays is usually used.
In capture ELISA kit, polystyrene microwell strips are pre-coated with antibodies directed to human IgM (anti-i chain). The patient’s serum/plasma sample is added, and during the first incubation, any IgM antibodies will be captured in the wells, and IgG antibodies on the other hand will not.
After washing out all other components of the sample and in particular IgG antibodies, the target IgM antibodies captured on the solid phase are detected by the addition of viral antigens conjugated to horseradish peroxidase (HRP). During the second incubation, the conjugated antigens will specifically react with the viral specific IgM antibodies.
After washing to remove unbound conjugates, Chromogen solutions are added to the wells. During the third incubation, the colorless chromogens are hydrolyzed by the bound HRP conjugate to a blue colored product. The blue color turns yellow after stopping the reaction with sulfuric acid, indicating a positive result; the amount of color can be measured and is proportional to the amount of antibody in the sample.
Wells containing samples negative for the target IgM antibodies remain colorless, indicating negative result.