HBeAb ELISA Kit

HBeAb ELISA Kit (乙肝e抗体HBeAb检测试剂盒) is a one-step, qualitative , enzyme-linked immunosorbent assay (ELISA) test kit, based on the Competitive Binding ELISA principle, which is used to detect HBV E-antigen Antibodies in human Serum or Plasma specimen.

Please refer to the Instruction for Use (IFU) of the HBeAb ELISA Kit.
(IFUs are subject to changes without prenotice; reading the package insert before carrying out any assay, it is always a good protocol for laboratory activity.)

Detailed information of HBeAb ELISA Kit

  1. General and Technical information of this HBeAb test kit;
  2. Packing specification of this Microwell style HBeAb test kit (gross weight and carton dimension etc.);
  3. Specimen used to carry out the assay, and the requirements on specimen collection, storage and processing; and,
  4. Specific immuno assay principle used in this Hepatitis B Test Kit.

Main technical information of this HBeAb Microwell test kit is listed in the following table:

Catalog No.:HBV 2314
Description:HBeAb ELISA Kit
Category:Hepatitis Disease
Style:Microwell
Unit:Box
Specimen:Serum, Plasma
No. of Step:One Step
Reading Time:60-15 Minutes
Cut Off:
Specification:48T/96T
Quan. or Qual.:Qualitative
Sensitivity:
Specificity:
Assay Principle:Competitive Binding ELISA

The standard kit size of Hepatitis B Microwell Test Kit is 96 Tests per box; 40 boxes per carton; 48T and 480T packing specifications are available for choice.


Tests per Kit:96 Tests
Dimension of Kit Box:13.5 * 9.0 * 8.0 CM
Boxes per Carton:40 Kits
G.W. per Carton:15.0 KG
Carton Dimension:59 * 45 * 38 CM
Carton Volume:0.100 CBM

Materials Provided

  • MICROWELL PLATE: 1 plate;
  • NEGATIVE CONTROL: 1 vial;
  • POSITIVE CONTROL SERUM: 1 vial;
  • SPECIMEN DILUENT: 1 vial;
  • HRP-CONJUGATE REAGENT: 1 vial;
  • WASH BUFFER: 1 bottle;
  • CHROMOGEN SOLUTION A: 1 vial;
  • CHROMOGEN SOLUTION B: 1 vial;
  • STOP SOLUTION: 1 vial;
  • PLASTIC SEALABLE BAG: 1 piece;
  • CARDBOARD PLATE COVER;
  • PACKAGE INSERT: 1 copy.

To carry out the assay with a HBeAb ELISA Kit, Serum or Plasma should be collected according to the instructions given in the Instruction For Use included in the test kit. After collecting, it is preferable to perform the testing as early as possible; if not viable, the specimen should be stored properly. A general introduction about the collection, and storage of the specimens is given below.

Plasma Collection

Collect blood specimen into a lavender, blue or green top blood collection tube, containing EDTA, citrate or heparin, respectively, by vein puncture.

Separate the plasma by centrifugation.

Carefully withdraw the plasma into a new pre-labeled tube.

Serum Collection

Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.

Allow the blood to clot, or separate the serum by centrifugation.

Carefully withdraw the serum into a new pre-labeled tube.

Serum, Plasma Storage Condition

Test specimens as soon as possible after collecting. The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.

Competitive Binding ELISA method is used in this HBeAb ELISA Kit to detect the HBV E-antigen Antibodies in human Serum or Plasma specifically. The introduction of this Competitive Binding ELISA testing principle is given blow.

ELISA Competitive Method Illustration

ELISA kit based on competitive principle is one-step test kit, in which a fixed amount of purified viral specific antigens is pre-coated in the microwell strips.

During the testing process, the sample and monoclonal antibodies conjugated to horseradish peroxidase (HRP) is added to the microwells together. During incubation, the target antibodies, if present in the sample, compete with the HRP conjugated antibodies to bind to the pre-coated antigens. When no target antibodies present in the sample, the HRP labeled antibodies will bind with the antigens inside the wells and any unbound HRP-Conjugate is removed during washing.

Chromogen A and B solutions are added into the wells and during the second incubation, the colorless Chromogens are hydrolyzed by the bound HRP-Conjugate to a blue colored product. The blue color turns yellow after stopping the reaction with sulfuric acid, indicating a NEGATIVE result.

No or low color developing suggests the presence of the target antibodies in the sample. So the color developed on the microwell strips is negatively related to the concentration of the target antibodies.