HBsAb Quantitative ELISA Kit
HBsAb Quantitative ELISA Kit (乙肝表面抗体定量诊断试剂盒) is a one-step, quantitative , enzyme-linked immunosorbent assay (ELISA) test kit, based on the Double Antigen Sandwich ELISA principle, which is used to detect Anti Hepatitis-B Surface antigen in human Serum or Plasma specimen.
Please refer to the Instruction for Use (IFU) of the HBsAb Quantitative ELISA Kit.
(IFUs are subject to changes without prenotice; reading the package insert before carrying out any assay, it is always a good protocol for laboratory activity.)
Detailed information of HBsAb Quantitative ELISA Kit
- General and Technical information of this HBsAb2 test kit;
- Packing specification of this Microwell style HBsAb2 test kit (gross weight and carton dimension etc.);
- Specimen used to carry out the assay, and the requirements on specimen collection, storage and processing; and,
- Specific immuno assay principle used in this Hepatitis B Test Kit.
Main technical information of this HBsAb Microwell test kit is listed in the following table:
|Catalog No.:||HBV 2224|
|Description:||HBsAb Quantitative ELISA Kit|
|No. of Step:||One Step|
|Reading Time:||60-15 Minutes|
|Quan. or Qual.:||Quantitative|
|Assay Principle:||Double Antigen Sandwich ELISA|
The standard kit size of Hepatitis B Microwell Test Kit is 96 Tests per box; 40 boxes per carton; 48T and 480T packing specifications are available for choice.
|Tests per Kit:||96 Tests|
|Dimension of Kit Box:||13.5 * 9.0 * 8.0 CM|
|Boxes per Carton:||40 Kits|
|G.W. per Carton:||15.0 KG|
|Carton Dimension:||59 * 45 * 38 CM|
|Carton Volume:||0.100 CBM|
- MICROWELL PLATE: 1 plate;
- NEGATIVE CONTROL: 1 vial;
- POSITIVE CONTROL SERUM: 1 vial;
- SPECIMEN DILUENT: 1 vial;
- HRP-CONJUGATE REAGENT: 1 vial;
- WASH BUFFER: 1 bottle;
- CHROMOGEN SOLUTION A: 1 vial;
- CHROMOGEN SOLUTION B: 1 vial;
- STOP SOLUTION: 1 vial;
- PLASTIC SEALABLE BAG: 1 piece;
- CARDBOARD PLATE COVER;
- PACKAGE INSERT: 1 copy.
To carry out the assay with a HBsAb Quantitative ELISA Kit, Serum or Plasma should be collected according to the instructions given in the Instruction For Use included in the test kit. After collecting, it is preferable to perform the testing as early as possible; if not viable, the specimen should be stored properly. A general introduction about the collection, and storage of the specimens is given below.
Collect blood specimen into a lavender, blue or green top blood collection tube, containing EDTA, citrate or heparin, respectively, by vein puncture.
Separate the plasma by centrifugation.
Carefully withdraw the plasma into a new pre-labeled tube.
Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.
Allow the blood to clot, or separate the serum by centrifugation.
Carefully withdraw the serum into a new pre-labeled tube.
Serum, Plasma Storage Condition
Test specimens as soon as possible after collecting. The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.
Double Antigen Sandwich ELISA method is used in this HBsAb Quantitative ELISA Kit to detect the Anti Hepatitis-B Surface antigen in human Serum or Plasma specifically. The introduction of this Double Antigen Sandwich ELISA testing principle is given blow.
In double antigen sandwich ELISA kit, a recombinant or purified antigen is pre-coated on the polystyrene microwell strips, which acts as the capturer. And, a second antigen conjugated to tracer enzyme -horseradish peroxidase (HRP) is used as the detector. Depending on whether the specimen is added together with the HRP conjugate into the microwell or not, double antigen sandwich ELSIA are subdivided to one-step kit and two-step kit.
In one step kit, the sample is incubated in the microwells together with the second antigen HRP conjugate. In case of presence of the target antibody in the sample, the pre-coated and conjugated antigens will be bound to the two variable domains of the antibody during incubation, thus the specific antigen-antibody-antigen-HRP immuno complex will develop and be captured on the solid phase.
After washing to remove unbound sample and conjugates, Chromogen solutions containing tetramethyl benzidine (TMB) and urea peroxide are added to the wells and in presence of the "sandwich" immuno complex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue colored product, which turns yellow after stopping the reaction with sulfuric acid, indicating a positive result. The amount of color can be measured and is proportional to the amount of antibody in the sample.
Wells containing samples negative for target antibody remain colorless.
For two-step kit, the antibodies in the sample are first allowed to react with the pre-coated antigens during the first incubation. During this process, the target antibodies, if present, will be captured on the polystyrene microwell strips. In turn, they are detected by the addition of the HRP-conjugated antigens, with the same color development as described for one-step kit.