HBsAg ELISA Kit (Two Step)
HBsAg ELISA Kit (Two Step) (乙肝表面抗原HBsAg试剂盒（两步法）) is a two-step, qualitative , enzyme-linked immunosorbent assay (ELISA) test kit, based on the Double Antibody Sandwich ELISA principle, which is used to detect Hepatitis B surface antigen in human Serum or Plasma specimen.
Please refer to the Instruction for Use (IFU) of the HBsAg ELISA Kit (Two Step).
(IFUs are subject to changes without prenotice; reading the package insert before carrying out any assay, it is always a good protocol for laboratory activity.)
Detailed information of HBsAg ELISA Kit (Two Step)
- General and Technical information of this HBsAg2 test kit;
- Packing specification of this Microwell style HBsAg2 test kit (gross weight and carton dimension etc.);
- Specimen used to carry out the assay, and the requirements on specimen collection, storage and processing; and,
- Specific immuno assay principle used in this Hepatitis B Test Kit.
Main technical information of this HBsAg Microwell test kit is listed in the following table:
|Catalog No.:||HBV 2124|
|Description:||HBsAg ELISA Kit (Two Step)|
|No. of Step:||Two Step|
|Reading Time:||60-30-30 Minutes|
|Quan. or Qual.:||Qualitative|
|Assay Principle:||Double Antibody Sandwich ELISA|
The standard kit size of Hepatitis B Microwell Test Kit is 96 Tests per box; 40 boxes per carton; 48T and 480T packing specifications are available for choice.
|Tests per Kit:||96 Tests|
|Dimension of Kit Box:||13.5 * 9.0 * 8.0 CM|
|Boxes per Carton:||40 Kits|
|G.W. per Carton:||15.0 KG|
|Carton Dimension:||59 * 45 * 38 CM|
|Carton Volume:||0.100 CBM|
- MICROWELL PLATE: 1 plate;
- NEGATIVE CONTROL: 1 vial;
- POSITIVE CONTROL SERUM: 1 vial;
- SPECIMEN DILUENT: 1 vial;
- HRP-CONJUGATE REAGENT: 1 vial;
- WASH BUFFER: 1 bottle;
- CHROMOGEN SOLUTION A: 1 vial;
- CHROMOGEN SOLUTION B: 1 vial;
- STOP SOLUTION: 1 vial;
- PLASTIC SEALABLE BAG: 1 piece;
- CARDBOARD PLATE COVER;
- PACKAGE INSERT: 1 copy.
To carry out the assay with a HBsAg ELISA Kit (Two Step), Serum or Plasma should be collected according to the instructions given in the Instruction For Use included in the test kit. After collecting, it is preferable to perform the testing as early as possible; if not viable, the specimen should be stored properly. A general introduction about the collection, and storage of the specimens is given below.
Collect blood specimen into a lavender, blue or green top blood collection tube, containing EDTA, citrate or heparin, respectively, by vein puncture.
Separate the plasma by centrifugation.
Carefully withdraw the plasma into a new pre-labeled tube.
Collect blood specimen into a red top blood collection tube by vein puncture, which contains no anticoagulants.
Allow the blood to clot, or separate the serum by centrifugation.
Carefully withdraw the serum into a new pre-labeled tube.
Serum, Plasma Storage Condition
Test specimens as soon as possible after collecting. The specimens can be stored up to 3 days at 2-8°C if not tested immediately. The specimens should be frozen at -20°C for longer time storage. Don’t freeze and thaw the specimens for many times. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.
Double Antibody Sandwich ELISA method is used in this HBsAg ELISA Kit (Two Step) to detect the Hepatitis B surface antigen in human Serum or Plasma specifically. The introduction of this Double Antibody Sandwich ELISA testing principle is given blow.
With this type of ELISA test kits, the detection target usually is hormone protein or viral antigen.
In the test kit, monoclonal or polyclonal antibodies specific to the target protein or antigen are used as capturer, which are pre-coated on the polystyrene microwell strips. The sample from the patient, such like urine, serum or plasma sample is added to the microwells. And a second antibody conjugated with horseradish peroxidase (HRP) is used as the detector, which then is added to the microwells. Usually, the samples and the second antibody conjugate are added to the microwell in the same time, thus it is a one-step assay process.
During incubation, the specific immuno-complex formed in case of presence of the target antigen or protein in the sample, and is captured on the solid phase.
After washing to remove sample serum proteins and unbound HRP-conjugate, Chromogen solutions containing tetramethyl benzidine (TMB) and urea peroxide are added to the wells. In presence of the antibody-antigen-antibody (HRP) "sandwich" immuno complex, the colorless Chromogens are hydrolyzed by the bounded HRP-conjugate to a blue colored product. The blue color turns yellow after stopping the reaction with sulfuric acid, indicating a positive result. The amount of color can be measured and is proportional to the amount of antigen or protein in the sample.
Wells containing samples negative for the target antigen or protein remain colorless.